John Augustus, Prince of Anhalt-Zerbst (Zerbst, 29 July 1677 – Zerbst, 7 November 1742), was a German prince of the House of Ascania and ruler of the principality of Anhalt-Zerbst.
He was the eldest son of Karl William, Prince of Anhalt-Zerbst, by his wife Sophie, daughter of August, Duke of Saxe-Weissenfels.
In 1718, after the death of his father, John Augustus succeeded him as prince of Anhalt-Zerbst.
John Augustus married Fredericka (b. Gotha, 24 March 1675; d. Karlsbad, 28 May 1709), daughter of Frederick I, Duke of Saxe-Gotha-Altenburg, on 25 May 1702 in Zerbst. They had no children. He was married for a second time to Hedwig Fredericka (b. Weiltingen, 18 October 1691; d. Zerbst, 14 August 1752), daughter of Frederick Ferdinand, Duke of Württemberg-Weiltingen, on 8 October 1715 in Zerbst. This union was also childless.
Since John Augustus died without issue, the elder line of Anhalt-Zerbst became extinct. On his death, he was succeeded by his cousins the princes of Anhalt-Dornburg.
| Preceded by Karl William |
Prince of Anhalt-Zerbst 1718–1742 |
Succeeded by John Louis II and Christian August |